mouse anti rabbit t lymphocytes fitc 1  (Bio-Rad)

Journal: Cell Reports Medicine

Article Title: Non-overlapping epitopes on the gHgL-gp42 complex for the rational design of a triple-antibody cocktail against EBV infection

doi: 10.1016/j.xcrm.2023.101296

Figure Lengend Snippet: 5E3 and the 5E3+6H2+10E4 cocktail provide potent protection against lethal EBV challenge in humanized mice (A) Experimental timeline for CD34 + hematopoietic stem cell (HSC) engraftment, antibody infusion, EBV challenge, and monitoring for various biological and clinical outcomes. A total of 400 μg of 5E3 (n = 6), 10E4 (n = 6), 5E3+6H2+10E4 cocktail (n = 6), AMMO1 (positive control, n = 5), and VRC01 (negative control, n = 5) was administered to NPI mice via intraperitoneal (i.p.) injection 24 h before intravenous (i.v.) challenge with Akata-EBV (25,000 GRUs). Uninfected control mice (n = 3) did not receive an injection of antibody or virus. Body weight, animal survival, EBV DNA copy number in the peripheral blood, and immunophenotype were recorded weekly for 10 weeks. Humanized mice were euthanized at week 10, and spleens were collected for further analysis. (B–D) Body weight (B), survival (C), and EBV DNA copy number in the peripheral blood samples (D) of mice were monitored weekly. Each line in (D) represents an individual mouse; the limit of detection (10 copies/μL) is indicated by a dashed line. Body weight was converted to percentage. (E–I) The percent changes in lymphocyte proportions of hCD45 + (E), hCD19 + (F), hCD3 + (G), hCD8 + (H), and hCD4 + (I) cells in the peripheral blood were analyzed using flow cytometry. All data are from one experimental replicate and presented as the mean ± SEM. Statistical analyses were performed using one-way ANOVA, comparing each group of data with that of the VRC01 group. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant. 5E3, 10E4, 5E3+6H2+10E4 combination, AMMO1, VRC01, and uninfected control are colored green, purple, deep pink, gray, brown, and black, respectively. See also Figure S3 .

Article Snippet: MOUSE ANTI RABBIT T LYMPHOCYTES:FITC , Bio-Rad , Cat# MCA800F; RRID: AB_321388.

Techniques: Positive Control, Negative Control, Injection, Control, Virus, Flow Cytometry

Journal: Cell Reports Medicine

Article Title: Non-overlapping epitopes on the gHgL-gp42 complex for the rational design of a triple-antibody cocktail against EBV infection

doi: 10.1016/j.xcrm.2023.101296

Figure Lengend Snippet:

Article Snippet: MOUSE ANTI RABBIT T LYMPHOCYTES:FITC , Bio-Rad , Cat# MCA800F; RRID: AB_321388.

Techniques: Marker, Virus, Recombinant, Adjuvant, Red Blood Cell Lysis, Reverse Transcription, Random Hexamer, Luciferase, Software, Cytometry